A specific, sensitive method for the determination of hyaluronate.
نویسندگان
چکیده
A sensitive and specific assay for hyaluronate was devised. Hyaluronate contained in biological mixtures was digested with a commercially available microbial hyaluronate lyase. The P-A4,5-eneglucopyranuronic acid residues contained at the nonreducing termini of the resulting oligosaccharides were oxidized with periodic acid to yield, among other products, formyl pyruvic acid. The latter compound reacted with thiobarbituric acid to yield a chromophore with an absorption maximum at 549 nm. Optimal conditions for quantitative assay of hyaluronate are described.
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ورودعنوان ژورنال:
- Analytical biochemistry
دوره 96 2 شماره
صفحات -
تاریخ انتشار 1979